Recombinant beta Catenin antibody

Catalog No. ABIN7650641

The Rabbit Monoclonal anti-beta Catenin antibody is suitable to detect beta Catenin in samples from Human. It has been validated for WB, IHC, FACS, IF and IHC (fp).

Quick Overview for Recombinant beta Catenin antibody (ABIN7650641)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CTNNB1-2030R

Product Details anti-beta Catenin Antibody

Purpose: Catenin, beta (CTNNB1) (CTNNB1/2030R)

Characteristics: Beta-catenin associates with the cytoplasmic portion of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion, including adherens junctions. Beta-catenin is involved in the Wnt signaling pathway as well as other signaling pathways, and is also found in complexes with many different proteins including the tumor suppressor protein APC. Defects in beta-catenin are associated with colorectal cancer, as well as many other cancer types. Beta-catenin is normally localized to the cell membrane, but can translocate to the nucleus in response to certain cell signaling. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human i²-catenin protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa or MCF-7 cells. Breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Beta-catenin

Background:

Synonyms: Beta-catenin, Catenin beta-1, Catenin (Cadherin associated protein), beta 1, CTNNB

Gene Symbol: CTNNB1

Tissue Expression: Epithelial cells

Molecular Weight: 92 kDa

Gene ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism