Cathepsin D antibody (AA 104-250) (CF®647)

Catalog No. ABIN7650658

This Mouse Monoclonal antibody specifically detects Cathepsin D in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Cathepsin D antibody (AA 104-250) (CF®647) (ABIN7650658)

Target: Cathepsin D (CTSD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cathepsin D antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CTSD-3275

Product Details anti-Cathepsin D Antibody (CF®647)

Binding Specificity: AA 104-250

Purpose: Cathepsin D (Tumor Marker)(CTSD/3275), CF647 conjugate

Characteristics: Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Cathepsin D protein (around aa 104-250)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MCF-7, K-562, A431 or SK-BR3 cells. Kidney, liver, lung or breast.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D

Background:

Synonyms: Cathepsin D, CTSD, Lysosomal aspartyl peptidase

Gene Symbol: CTSD

Molecular Weight: 46 kDa (Procathepsin D), 28 kDa (Cathepsin D)

Gene ID: 1509

UniProt: P07339

Pathways: Peptide Hormone Metabolism