Recombinant CD207 antibody (AA 74-213) (CF®640R)

Catalog No. ABIN7650953

The Mouse Monoclonal anti-CD207 antibody has been validated for . It is suitable to detect CD207 in samples from Human.

Quick Overview for Recombinant CD207 antibody (AA 74-213) (CF®640R) (ABIN7650953)

Target: CD207 (CD207 Molecule, Langerin (CD207))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD207 antibody is conjugated to CF®640R

Application: Please inquire

Clone: RLGRN-1821

Product Details anti-CD207 Antibody (CF®640R)

Binding Specificity: AA 74-213

Purpose: Langerin / CD207 (Marker of Langerhans Cells)(rLGRN/1821), CF640R conjugate

Characteristics: Langerhans cells (LCs) are a subset of immature dendritic cells (DCs) that specifically localize in the epidermis and other mucosal epithelia. Epidermal LCs possess strong immuno-stimulatory capacity and play a central role in the initiation and regulation of immune responses. Langerin (CD207) is a Ca2 -dependent, C-type lectin domain containing, type II transmembrane protein that induces epidermal LCs to differentiate into Birbeck granules (BG). BGs are organelles with superimposing and zippering membranes that influence proper class I type antigen presentation to the circulating T cells. Human spleen, lymph node, thymus, liver, lung and heart express Langerin protein. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa74-213) of human Langerin protein

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HeLa or A549 cells (WB).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD207

Target: CD207 (CD207 Molecule, Langerin (CD207))

Alternative Name: CD207

Background:

Synonyms: C-type lectin domain family 4 member K, CD207, CLEC4K, Langerhans cell specific c type lectin, Langerin

Gene Symbol: CD207

Tissue Expression: Heart|Lymph node|Dendritic cells|Liver|Lung|Spleen|Thymus

Molecular Weight: 48 kDa

Gene ID: 50489

UniProt: Q9UJ71