Recombinant CD34 antibody (CF®740)

Catalog No. ABIN7651289

The Rabbit Monoclonal anti-CD34 antibody is suitable to detect CD34 in samples from Human and Rat. It has been validated for FACS, IHC and IHC (fp).

Quick Overview for Recombinant CD34 antibody (CF®740) (ABIN7651289)

Target: CD34

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: HPCA1-1806R

Product Details anti-CD34 Antibody (CF®740)

Purpose: CD34 (Hematopoietic Stem Cell & Endothelial Marker) (HPCA1/1806R), CF740 conjugate

Characteristics: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human HPCA1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology (formalin) 2-4 μg/mL. Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: KG-1 cells, Tonsil, or Angiosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Synonyms: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Gene Symbol: CD34

Tissue Expression: Endothelial cells|Hematopoietic stem cells

Molecular Weight: 90-110 kDa

Gene ID: 947

UniProt: P28906