CD40 Ligand antibody (AA 108-261) (CF®640R)

Catalog No. ABIN7651442

The Mouse Monoclonal anti-CD40 Ligand antibody has been validated for FACS, IHC, IF and IHC (fp). It is suitable to detect CD40 Ligand in samples from Human.

Quick Overview for CD40 Ligand antibody (AA 108-261) (CF®640R) (ABIN7651442)

Target: CD40 Ligand (CD40LG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is conjugated to CF®640R

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CD40LG-2761

Product Details anti-CD40 Ligand Antibody (CF®640R)

Binding Specificity: AA 108-261

Purpose: CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)(CD40LG/2761), CF640R conjugate

Characteristics: CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 108-261) of human CD40LG protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Jurkat cells. Tonsil, Spleen or Thymus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154

Background:

Synonyms: CD154, gp39, hCD40L, HIGM1, IGM, IMD3, T B cell activating molecule, T BAM, T-cell antigen Gp39, TNF-related activation protein, TrAP, Tumor necrosis factor ligand superfamily member 5 (TNFSF5)

Gene Symbol: CD40LG

Molecular Weight: 36 kDa

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints