Recombinant CD59 antibody (CF®488A)

Catalog No. ABIN7651714

This Rabbit Monoclonal antibody specifically detects CD59 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant CD59 antibody (CF®488A) (ABIN7651714)

Target: CD59

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD59 antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MACIF-2867R

Product Details anti-CD59 Antibody (CF®488A)

Purpose: CD59 / Complement Regulatory Protein / Protectin (MACIF/2867R), CF488A conjugate

Characteristics: This antibody reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI, Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CD59 protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with baboon or horse. Flow cytometry: 0.5-1 μg/million cells. Functional Studies Order Ab without Azide. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background:

Synonyms: 20 kDa homologous restriction factor (HRF20), Complement regulatory protein, Human leukocyte antigen MIC11, MAC-inhibitory protein (MACIP), Membrane attack complex inhibition factor (MACIF), Membrane inhibitor of reactive lysis, MIRL, MSK21, Protectin, T cell activating protein

Gene Symbol: CD59

Molecular Weight: 20 kDa

Gene ID: 966

UniProt: P13987

Pathways: Complement System