CD7 antibody (Biotin)

Catalog No. ABIN7651945

This Mouse Monoclonal antibody specifically detects CD7 in IF. It exhibits reactivity toward Human.

Quick Overview for CD7 antibody (Biotin) (ABIN7651945)

Target: CD7

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD7 antibody is conjugated to Biotin

Application: Immunofluorescence (IF)

Clone: HuLy-m2

Product Details anti-CD7 Antibody (Biotin)

Purpose: CD7 (T-Cell Leukemia Marker)(HuLy-m2), Biotin conjugate

Characteristics: Recognizes a protein of 40 kDa, identified as CD7 (Workshop IV, Code T165). CD7 is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.

Immunogen: Human thymocytes

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Jurkat, HUT-78, Molt-4, CEM cells, or human peripheral blood lymphocytes. Lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD7

Target: CD7

Alternative Name: CD7

Background:

Synonyms: GP40, Leu9, p41, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41

Gene Symbol: CD7

Tissue Expression: NK cells|T-cells

Molecular Weight: 40 kDa

Gene ID: 924

UniProt: P09564

Pathways: Cell-Cell Junction Organization