CD81 antibody (ExoBrite™ 800)

Catalog No. ABIN7652106

The ExoBrite™ 800-conjugated Mouse Monoclonal anti-CD81 antibody (ABIN7652106) specifically detects CD81 in WB and ExoS. The antibody is reactive with Human, Non-Human Primate, Cynomolgus and Baboon samples.

Quick Overview for CD81 antibody (ExoBrite™ 800) (ABIN7652106)

Target: CD81

Reactivity: Human, Non-Human Primate, Cynomolgus, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is conjugated to ExoBrite™ 800

Application: Western Blotting (WB), Exosome Staining (ExoS)

Product Details anti-CD81 Antibody (ExoBrite™ 800)

Purpose: ExoBrite™ 770/800 CD81 Western Antibody (100 tests)

Characteristics: ExoBrite™ CD81 Western Antibody is optimized for detection of the exosome marker CD81 in isolated EV extracts by near-IR (NIR) fluorescent WB. ExoBrite™ Western 770/800 conjugates have Ex/Em at 770/797 nm for detection in the 800 nm channel. Supplied as 1 mL of 100 μg/mL for 100 mini-blots.

Isotype: IgG1, kappa

Application Details

Application Notes:

Suggested Antibody Volume per Assay: 10 uL/test

Product features: Designed for western blot detection, validated with cell and exosome lysates

Application Notes: Recommended amount for western blot: 100 ng/mL = 1:1000 dilution. Optimal concentration to be determined by end-user.

Comment:

Positive Control: MCF-7 cells | MCF-7 derived exosomes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Proprietary buffer containing 0.05 % sodium azide

Preservative: Other preservative, Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light,

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD81

Target: CD81

Alternative Name: CD81

Background:

Synonyms: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28

Keywords: exosome, vesicle, western, exobrite

Gene Symbol: CD81

Tissue Expression: Exosomes | Lymphocytes | Monocytes/macrophages | Hepatocytes

Molecular Weight: 26 kDa

Gene ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C