CD9 antibody (ExoBrite™ 700)

Catalog No. ABIN7652282

The Mouse Monoclonal anti-CD9 antibody is suitable to detect CD9 in samples from Human, Cow, Dog, Horse, Rabbit, Sheep, Non-Human Primate, Cynomolgus and Baboon. It has been validated for WB and ExoS.

Quick Overview for CD9 antibody (ExoBrite™ 700) (ABIN7652282)

Target: CD9

Reactivity: Human, Cow, Dog, Horse, Rabbit, Sheep, Non-Human Primate, Cynomolgus, Baboon

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is conjugated to ExoBrite™ 700

Application: Western Blotting (WB), Exosome Staining (ExoS)

Product Details anti-CD9 Antibody (ExoBrite™ 700)

Purpose: ExoBrite™ 680/700 CD9 Western Antibody (100 tests)

Characteristics: ExoBrite™ CD9 Western Antibody is optimized for detection of the exosome marker CD9 in isolated EV extracts by near-IR (NIR) fluorescent WB. ExoBrite™ Western 680/700 conjugates have Ex/Em at 681/698 nm for detection in the 700 nm channel. Supplied as 1 mL of 100 μg/mL for 100 mini-blots.

Isotype: IgG1, kappa

Application Details

Application Notes:

Suggested Antibody Volume per Assay: 10 uL/test

Product features: Designed for western blot detection, validated with cell and exosome lysates

Application Notes: Recommended amount for western blot: 100 ng/mL = 1:1000 dilution. Optimal concentration to be determined by end-user.

Comment:

Positive Control: MCF-7 cells | MCF-7 derived exosomes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Proprietary buffer containing 0.05 % sodium azide

Preservative: Other preservative, Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light,

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background:

Synonyms: Tetraspanin-29 (TSPAN29), BA-2/p24 antigen, BA2, BTCC1, CD9, Cell growth-inhibiting gene 2 protein, DRAP27, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein (MRP1), p24

Keywords: exosome, vesicle, western, exobrite

Gene Symbol: CD9

Tissue Expression: Exosomes | Platelets | Basophils | Eosinophils | Lymphocytes |Epithelial cells

Molecular Weight: 24 kDa

Gene ID: 928

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization