Chp2 antibody (CF®488A)

Catalog No. ABIN7652374

This Mouse Monoclonal antibody specifically detects Chp2 in WB and IF. It exhibits reactivity toward Human.

Quick Overview for Chp2 antibody (CF®488A) (ABIN7652374)

Target: Chp2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chp2 antibody is conjugated to CF®488A

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: CPTC-CHP2-1

Product Details anti-Chp2 Antibody (CF®488A)

Purpose: Calcineurin B homologous protein 2 / HCC Antigen 520(CPTC-CHP2-1), CF488A conjugate

Characteristics: Calcineurin B homologous protein 2, also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na/H exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CHP2 protein

Isotype: IgG2c, kappa

Application Details

Application Notes: Western blot 1-2 μg/mL, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: MCF-7, PC-3, HT29, K562 or Jurkat cell lysates. Human appendix or testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Chp2

Target: Chp2

Alternative Name: Calcineurin B homologous protein 2

Background:

Synonyms: Calcineurin B homologous protein 2, Calcineurin-like EF hand protein 2, HCA520, hepatocellular carcinoma antigen gene 520

Gene Symbol: CHP2

Molecular Weight: 22 kDa

Gene ID: 63928

UniProt: O43745