Chp2 antibody

Catalog No. ABIN6940567

The Mouse Monoclonal anti-Chp2 antibody (Clone CPTC-CHP2-1) (ABIN6940567) specifically detects Chp2 in WB, IF and IHC. The antibody is reactive with Human samples.

Quick Overview for Chp2 antibody (ABIN6940567)

Target: Chp2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chp2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: CPTC-CHP2-1

Product Details anti-Chp2 Antibody

Purpose: Calcineurin B homologous protein 2 / HCC Antigen 520 Antibody

Specificity: CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length CHP2 protein

Isotype: IgG2c, kappa

Application Details

Application Notes:

Positive Control: MCF-7, PC-3, HT29, K562 or Jurkat cell lysates. Human appendix or testis.

Known Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Chp2

Target: Chp2

Background:

calcineurin-like EF hand protein 2, calcineurin-like EF-hand protein 2, HCA520, hepatocellular carcinoma antigen gene 520, Hepatocellular carcinoma-associated antigen 520 homolog

K562 or Jurkat cell lysates. Human appendix or testis., MCF-7

Molecular Weight: 22kDa

Gene ID: 63928, 178589

UniProt: O43745