Clathrin Heavy Chain (CLTC) antibody (CF®640R)

Catalog No. ABIN7652444

The Mouse Monoclonal anti- antibody has been validated for IF. It is suitable to detect in samples from Human, Mouse, Rat, Cow, Pig, Dog, Xenopus laevis, Avian and Green Monkey.

Quick Overview for Clathrin Heavy Chain (CLTC) antibody (CF®640R) (ABIN7652444)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Xenopus laevis, Avian, Green Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®640R

Application: Immunofluorescence (IF)

Clone: CHC-1432

Product Details

Purpose: Clathrin, Heavy Chain(CHC/1432), CF640R conjugate

Characteristics: Recognizes protein of 192 kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Clathrin Heavy Chain protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: HeLa cells. Placenta or Prostate Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin, Heavy Chain

Background:

Synonyms: Clathrin heavy chain, Clathrin heavy chain 1, Clathrin heavy chain 2, Clathrin heavy chain on chromosome 17 (CHC17 or CHC-17), CLH22, CLTC, CLTCL, CLTCL1, CLTCL2, CLTD

Gene Symbol: CLTC

Molecular Weight: 192 kDa

Gene ID: 1213

UniProt: P53675, Q00610