GM-CSF antibody (CF®740)
Quick Overview for GM-CSF antibody (CF®740) (ABIN7652488)
Target
See all GM-CSF (CSF2) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- GM-CSF(BVD2-21C11), CF740 conjugate
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Characteristics
- Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Immunogen
- Recombinant human GM-CSF protein
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Isotype
- IgG2a, kappa
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Application Notes
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (frozen) 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Neutralization Studies order Ab without azide. Optimal dilution for a specific application should be determined by user
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Comment
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Positive Control: Lymph node and tonsil
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.1 mg/mL
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Buffer
- PBS, 0.1 % rBSA, 0.05 % azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Protect from light
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Storage
- 4 °C
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Storage Comment
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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Expiry Date
- 24 months
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Alternative Name
- GM-CSF
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Background
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Synonyms: Burst Promoting Activity, Eosinophil Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim
Gene Symbol: CSF2
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Molecular Weight
- 22 kDa
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Gene ID
- 1437
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UniProt
- P04141
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Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
Target
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