Recombinant CD21 antibody (AA 142-240) (CF®594)

Catalog No. ABIN7652550

The CF®594-conjugated Rabbit Monoclonal anti-CD21 antibody (Clone CR2-3124R) (ABIN7652550) specifically detects CD21 in FACS, IHC, IF and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for Recombinant CD21 antibody (AA 142-240) (CF®594) (ABIN7652550)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD21 antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CR2-3124R

Product Details anti-CD21 Antibody (CF®594)

Binding Specificity: AA 142-240

Purpose: CD21 (Mature B-Cell & Follicular Dendritic Cell Marker)(CR2/3124R), CF594 conjugate

Characteristics: Recognizes a protein of 140 kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MOLT4, WEHI-231 cells and Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Background:

Synonyms: CD21, Complement C3d receptor (C3DR), Complement Receptor type 2 (CR2), CVID7, EBV receptor, EBV-R, Epstein-Barr virus receptor, EVBR, SLEB9

Gene Symbol: CR2

Tissue Expression: Antigen presenting cells|B-cells|Lymphocytes

Molecular Weight: 140 kDa

Gene ID: 1380

UniProt: P20023

Pathways: Complement System