CD35 antibody (CF®568)

Catalog No. ABIN7652668

This Mouse Monoclonal antibody specifically detects CD35 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for CD35 antibody (CF®568) (ABIN7652668)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: To5

Product Details anti-CD35 Antibody (CF®568)

Purpose: CD35 / CR1 (Follicular Dendritic Cell Marker)(To5), CF568 conjugate

Characteristics: Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21 /CD35 /CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CR1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Follicular dendritic cells (FDC) in tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background:

Synonyms: C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen

Gene Symbol: CR1

Tissue Expression: Antigen presenting cells

Molecular Weight: 210-220 kDa

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity