Cyclin B1 antibody (CF®740)

Catalog No. ABIN7652735

The Mouse Monoclonal anti-Cyclin B1 antibody is suitable to detect Cyclin B1 in samples from Human, Mouse and Hamster. It has been validated for IF and FACS.

Quick Overview for Cyclin B1 antibody (CF®740) (ABIN7652735)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is conjugated to CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: V92-1

Product Details anti-Cyclin B1 Antibody (CF®740)

Purpose: Cyclin B1(V92.1), CF740 conjugate

Characteristics: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Hamster Cyclin B1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

Synonyms: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Gene Symbol: CCNB1

Molecular Weight: 55-62 kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase