Cyclin E1 antibody (AA 10-176) (CF®594)

Catalog No. ABIN7652798

The Mouse Monoclonal anti-Cyclin E1 antibody has been validated for . It is suitable to detect Cyclin E1 in samples from Human.

Quick Overview for Cyclin E1 antibody (AA 10-176) (CF®594) (ABIN7652798)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is conjugated to CF®594

Application: Please inquire

Clone: CCNE1-2587

Product Details anti-Cyclin E1 Antibody (CF®594)

Binding Specificity: AA 10-176

Purpose: Cyclin E (G1/S-Phase Cyclin)(CCNE1/2587), CF594 conjugate

Characteristics: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176)

Isotype: IgG, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HeLa Cells. Breast or Colon Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E

Background:

Synonyms: CCNE1, Cyclin E1, G1/S-specific cyclin-E1

Gene Symbol: CCNE1

Molecular Weight: 53 kDa

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases