Recombinant Cytochrome C antibody (AA 1-80) (CF®640R)

Catalog No. ABIN7652961

This anti-Cytochrome C antibody is a Mouse Monoclonal antibody detecting Cytochrome C in WB, IHC, FACS and IHC (fp). Suitable for Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster and Frog.

Quick Overview for Recombinant Cytochrome C antibody (AA 1-80) (CF®640R) (ABIN7652961)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCYCS-1010

Product Details anti-Cytochrome C Antibody (CF®640R)

Binding Specificity: AA 1-80

Purpose: Cytochrome C (Mitochondrial Marker)(rCYCS/1010), CF640R conjugate

Characteristics: Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome c

Background:

Synonyms: CYC, CYCS, HCS, THC4

Gene Symbol: CYCS

Molecular Weight: 15 kDa

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity