Cytochrome C antibody

Catalog No. ABIN7652995

This Mouse Monoclonal antibody specifically detects Cytochrome C in FACS and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Cytochrome C antibody (ABIN7652995)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6H2-B4

Product Details anti-Cytochrome C Antibody

Purpose: Cytochrome C (Mitochondrial Marker)(6H2.B4)

Characteristics: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Immunogen: Rat full-length cytochrome c protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome c

Background:

Synonyms: CYC, CYCS, HCS, THC4

Gene Symbol: CYCS

Molecular Weight: 15 kDa

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity