Recombinant Desmin antibody (CF®647)

Catalog No. ABIN7653076

This Rabbit Monoclonal antibody specifically detects Desmin in IHC and IHC (fp). It exhibits reactivity toward Human, Rat, Chicken and Hamster.

Quick Overview for Recombinant Desmin antibody (CF®647) (ABIN7653076)

Target: Desmin (DES)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Chicken, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Desmin antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: DES-2960R

Product Details anti-Desmin Antibody (CF®647)

Purpose: Desmin (Muscle Cell Marker) (DES/2960R), CF647 conjugate

Characteristics: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alphalpha-helicalcoiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. The antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human DES protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: SJRH30 Cells. Uterus or Leiomyosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background:

Synonyms: CMD1I, CSM1, CSM2, DES, Intermediate filament protein

Gene Symbol: DES

Tissue Expression: Muscle

Molecular Weight: 52 kDa

Gene ID: 1674

UniProt: P17661