Desmoglein 3 antibody (AA 379-491) (CF®640R)

Catalog No. ABIN7653124

This Mouse Monoclonal antibody specifically detects Desmoglein 3 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Desmoglein 3 antibody (AA 379-491) (CF®640R) (ABIN7653124)

Target: Desmoglein 3 (DSG3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 3 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: DSG3-2837

Product Details anti-Desmoglein 3 Antibody (CF®640R)

Binding Specificity: AA 379-491

Purpose: Desmoglein-3 (Squamous Cell Marker)(DSG3/2837), CF640R conjugate

Characteristics: Recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80 % ) and specificity (100 %) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 379-491) human DSG3 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A431 cells. Skin or Lung Squamous Cell Carcinoma (SCC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Desmoglein 3

Target: Desmoglein 3 (DSG3)

Alternative Name: Desmoglein-3

Background:

Synonyms: 130 kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)

Gene Symbol: DSG3

Tissue Expression: Epidermal cells

Molecular Weight: 130 kDa

Gene ID: 1830

UniProt: P32926