DMC1 antibody

Catalog No. ABIN7653217

This Mouse Monoclonal antibody specifically detects DMC1 in IHC and IHC (fp). It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for DMC1 antibody (ABIN7653217)

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This DMC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 2H12-4

Product Details anti-DMC1 Antibody

Purpose: DMC1(2H12/4)

Characteristics: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the doublestrand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Nibrin and Dmc1. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients. Dmc1 is specifically involved in meiotic recombination. An alternative spliced form of Dmc1, designated Dmc1-D, is deleted for a region between the two motifs involved in nucleotide binding. The alternatively spliced Dmc1-D transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human DMC1 protein.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa cells. Cervix or prostate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for DMC1

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Alternative Name: DMC1

Background:

Synonyms: Disrupted meiotic cDNA 1 homolog, dJ199H16.1, DMC1 dosage suppressor of mck1 homolog, DMC1H, DNA meiotic recombinase 1, HsLim15, LIM15, Meiotic recombination protein DMC1/LIM15 homolog, MGC150472, MGC150473

Gene Symbol: DMC1

Molecular Weight: 37 kDa

Gene ID: 11144

UniProt: Q14565