Geminin antibody (Biotin)

Catalog No. ABIN7653265

This Mouse Monoclonal antibody specifically detects Geminin in IF. It exhibits reactivity toward Human.

Quick Overview for Geminin antibody (Biotin) (ABIN7653265)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Geminin antibody is conjugated to Biotin

Application: Immunofluorescence (IF)

Clone: CPTC-GMMN-1

Product Details anti-Geminin Antibody (Biotin)

Purpose: Geminin / DNA Replication Inhibitor(CPTC-GMMN-1), Biotin conjugate

Characteristics: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphasepromoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitinmediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HEK293, HeLa, MCF-7, Tonsil, Triple-negative breast cancer.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: DNA Replication Inhibitor

Background:

Synonyms: DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GMMN, RP3 369A17.3

Gene Symbol: GMMN

Molecular Weight: 35 kDa

Gene ID: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication