EGLN1 antibody (AA 1-24) (Biotin)

Catalog No. ABIN7653372

This anti-EGLN1 antibody is a Mouse Monoclonal antibody detecting EGLN1 in . Suitable for Human.

Quick Overview for EGLN1 antibody (AA 1-24) (Biotin) (ABIN7653372)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGLN1 antibody is conjugated to Biotin

Application: Please inquire

Clone: 366G-76-3

Product Details anti-EGLN1 Antibody (Biotin)

Binding Specificity: AA 1-24

Purpose: EGLN1 / PHD2(366G/76/3), Biotin conjugate

Characteristics: This antibody recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Residues 1-24 of PHD2

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: MCF7 cells. Brain or adrenal gland.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background:

Synonyms: Chromosome 1 Open Reading Frame 12 (C1ORF12), DKFZp761F179, ECYT3, Egl 9 family hypoxia inducible factor 1, Egl nine homolog 1, Egln1, HIF Prolyl Hydroxylase 2 (HIF PH2), HIFPH2, HPH2, Hypoxia inducible factor prolyl hydroxylase 2, ORF13, P4H2, PhD2, PNAS 118, PNAS 137, Prolyl hydroxylase domain-containing protein 2, SM20, Zinc finger MYND domain containing protein 6 (ZMYND6)

Gene Symbol: EGLN1

Molecular Weight: 46 kDa

Gene ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect