Endoglin antibody (CF®647)

Catalog No. ABIN7653474

This anti-Endoglin antibody is a Mouse Monoclonal antibody detecting Endoglin in FACS and IF. Suitable for Human, Rat, Pig, Horse and Monkey.

Quick Overview for Endoglin antibody (CF®647) (ABIN7653474)

Target: Endoglin (ENG)

Reactivity: Human, Rat, Pig, Horse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ENG-1621

Product Details anti-Endoglin Antibody (CF®647)

Purpose: Endoglin / CD105 (ENG/1621), CF647 conjugate

Characteristics: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for prostate adenocarcinoma, and cancers of the lung, stomach, breast, and brain. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant extracellular fragment of human Endoglin protein

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 1-2 μg/million cells. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 2-4 μg/mL. ELISA: For coating order antibody without BSA. For functional studies, order Ab without azide. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, A431 and HL-60 cells. Colon.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105

Background:

Synonyms: CD105, END, Endoglin, Eng, HHT1, S-endoglin

Gene Symbol: ENG

Tissue Expression: Endothelial cells

Molecular Weight: 84 kDa

Gene ID: 2022

UniProt: P17813