EZH2 antibody (CF®740)

Catalog No. ABIN7653494

The Mouse Monoclonal anti-EZH2 antibody has been validated for WB. It is suitable to detect EZH2 in samples from Human.

Quick Overview for EZH2 antibody (CF®740) (ABIN7653494)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EZH2 antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: EZH2-2536

Product Details anti-EZH2 Antibody (CF®740)

Purpose: EZH2 / KMT6(EZH2/2536), CF740 conjugate

Characteristics: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human EZH2 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: MCF7, HeLa, U-2 OS or HEK-293 whole cell lysates. Human tonsil, testis or prostate tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background:

Synonyms: Enhancer of zeste 2 polycomb repressive complex 2 subunit, Enhancer of zeste homolog 2 (EZH2), ENX1, Enx1h, EZH2b, Histone-lysine N-methyltransferase EZH2, KMT6, KMT6A, Lysine N-methyltransferase 6, WVS, WVS2

Gene Symbol: EZH2

Molecular Weight: 81-102 kDa

Gene ID: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation