Cytokeratin 7 antibody (AA 251-350)

Catalog No. ABIN732023

The Rabbit Polyclonal anti-Cytokeratin 7 antibody has been validated for WB, FACS, IHC (p), ELISA, IHC (fro), IF (cc) and IF (p). It is suitable to detect Cytokeratin 7 in samples from Human and Mouse. There are 6+ publications available.

Quick Overview for Cytokeratin 7 antibody (AA 251-350) (ABIN732023)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cytokeratin 7 Antibody

Binding Specificity: AA 251-350

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human of human CK7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cytokeratin 7 antibody (ABIN732023)

Hu, Li, Zhang, Zheng, Wang, Zhang, Zhang, Gu, Ye, Guo, Yang, Wang: "Phosphoinositide 3-Kinase (PI3K) Subunit p110δ Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse." in: Scientific reports, Vol. 6, pp. 28201, (2018) (PubMed).

Uchikura, Matsubara, Muto, Matsubara, Fujioka, Matsumoto, Sugiyama: "Extranuclear Translocation of High-Mobility Group A1 Reduces the Invasion of Extravillous Trophoblasts Involved in the Pathogenesis of Preeclampsia." in: Reproductive sciences (Thousand Oaks, Calif.), pp. 1933719117697250, (2017) (PubMed).

Matsubara, Matsubara, Mori, Uchikura, Hamada, Fujioka, Hashimoto, Matsumoto: "Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice." in: Hypertension research : official journal of the Japanese Society of Hypertension, Vol. 39, Issue 6, pp. 407-14, (2017) (PubMed).

Ding, Liu, Chang, Chu: "Expression of CD133 in endometrial cancer cells and its implications." in: Journal of Cancer, Vol. 8, Issue 11, pp. 2142-2153, (2017) (PubMed).

Hu, Zhou, Gao, Liu, Yan, Zou, Chen: "IFN-? inhibits osteopontin expression in human decidual stromal cells and can be attenuated by 1?,25-dihydroxyvitamin D3." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 68, Issue 4, pp. 353-61, (2012) (PubMed).

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: CK7

Background:

Synonyms: K7, CK7, SCL, K2C7, Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, KRT7

Background: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Gene ID: 3855

UniProt: P08729