Fibronectin antibody (CF®740)

Catalog No. ABIN7654049

The Mouse Monoclonal anti-Fibronectin antibody has been validated for WB, IHC, IF, ELISA, IHC (fp) and Func. It is suitable to detect Fibronectin in samples from Human.

Quick Overview for Fibronectin antibody (CF®740) (ABIN7654049)

Target: Fibronectin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Functional Studies (Func)

Clone: HFN7-1

Product Details anti-Fibronectin Antibody (CF®740)

Purpose: Fibronectin(HFN7.1), CF740 conjugate

Characteristics: Fibronectin is a dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: SW156 cells or Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Fibronectin

Target: Fibronectin

Background:

Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

Gene Symbol: FN1

Molecular Weight: 220 kDa (monomer), 440 kDa (dimer)

Gene ID: 2335

UniProt: P02751