Fibronectin antibody (AA 467-595) (Biotin)

Catalog No. ABIN7654061

The Mouse Monoclonal anti-Fibronectin antibody has been validated for . It is suitable to detect Fibronectin in samples from Human.

Quick Overview for Fibronectin antibody (AA 467-595) (Biotin) (ABIN7654061)

Target: Fibronectin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is conjugated to Biotin

Application: Please inquire

Clone: FN1-2949

Product Details anti-Fibronectin Antibody (Biotin)

Binding Specificity: AA 467-595

Purpose: Fibronectin(FN1/2949), Biotin conjugate

Characteristics: Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 467-595) of human fibronectin protein

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: SW156 cells or Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Fibronectin

Target: Fibronectin

Background:

Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

Gene Symbol: FN1

Molecular Weight: 220 kDa (monomer), 440 kDa (dimer)

Gene ID: 2335

UniProt: P02751