Filaggrin antibody (AA 998-1104) (CF®405S)

Catalog No. ABIN7654068

This anti-Filaggrin antibody is a Mouse Monoclonal antibody detecting Filaggrin in IHC and IHC (fp). Suitable for Human.

Quick Overview for Filaggrin antibody (AA 998-1104) (CF®405S) (ABIN7654068)

Target: Filaggrin (FLG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: FLG-1563

Product Details anti-Filaggrin Antibody (CF®405S)

Binding Specificity: AA 998-1104

Purpose: Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate

Characteristics: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Filaggrin protein fragment (around aa 998-1104)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: U-251-MG cells. Skin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: Filaggrin

Background:

Synonyms: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin

Gene Symbol: FLG

Molecular Weight: 26-45 kDa (Processed), 350 kDa (Profilaggrin)

Gene ID: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance