FLI1 antibody (AA 107-261) (CF®740)

Catalog No. ABIN7654221

This anti-FLI1 antibody is a Mouse Monoclonal antibody detecting FLI1 in WB. Suitable for Human.

Quick Overview for FLI1 antibody (AA 107-261) (CF®740) (ABIN7654221)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLI1 antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: FLI1-1312

Product Details anti-FLI1 Antibody (CF®740)

Binding Specificity: AA 107-261

Purpose: FLI1 (Ewing s Sarcoma & Endothelial Marker) (FLI1/1312), CF740 conjugate

Characteristics: This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa 107-261) of human FLI1 protein

Isotype: IgG

Application Details

Application Notes: Western blotting 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background:

Synonyms: ERGB transcription factor, Ewing Sarcoma breakpoint region 2 (EWSR2), FLI1, FLI1 EWS fusion gene, Friend leukemia integration 1 (FLI1) transcription factor, Friend leukemia virus integration 1, Proto-oncogene Fli-1, SIC1, Transcription factor ERGB, Viral integration region FLI1

Gene Symbol: FLI1

Tissue Expression: Endothelial cells

Molecular Weight: 51 kDa

Gene ID: 2313

UniProt: Q01543