Recombinant GP2 antibody (AA 35-179) (Biotin)

Catalog No. ABIN7654572

The Biotin-conjugated Rabbit Monoclonal anti-GP2 antibody (Clone GP2-2569R) (ABIN7654572) specifically detects GP2 in WB, IHC and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for Recombinant GP2 antibody (AA 35-179) (Biotin) (ABIN7654572)

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: GP2-2569R

Product Details anti-GP2 Antibody (Biotin)

Binding Specificity: AA 35-179

Purpose: GP2 (Glycoprotein 2) / ZAP75 (GP2/2569R), Biotin conjugate

Characteristics: GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GP2 protein fragment (around aa35-179)

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with mouse. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: PANC-1 cells. Pancreas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GP2

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Alternative Name: GP2

Target Type: Viral Protein

Background:

Synonyms: Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75

Gene Symbol: GP2

Tissue Expression: M cells

Molecular Weight: 59 kDa

Gene ID: 2813

UniProt: P55259