Recombinant ICAM-3/CD50 antibody (CF®405S)

Catalog No. ABIN7655556

This anti-ICAM-3/CD50 antibody is a Rabbit Monoclonal antibody detecting ICAM-3/CD50 in IHC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant ICAM-3/CD50 antibody (CF®405S) (ABIN7655556)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ICAM3-2873R

Product Details anti-ICAM-3/CD50 Antibody (CF®405S)

Purpose: CD50 / ICAM3(ICAM3/2873R), CF405S conjugate

Characteristics: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human ICAM3 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: CD50

Background:

Synonyms: CD50, CDW 50, CDw50, ICAM 3, ICAM-3, ICAM-R, ICAM3, ICAM3_HUMAN, ICAMR, Intercellular adhesion molecule 3

Gene Symbol: ICAM3

Tissue Expression: Lymphocytes|T-cells

Molecular Weight: 110-160 kDa

Gene ID: 3385

UniProt: P32942