Recombinant IL3RA antibody (AA 26-171) (CF®568)

Catalog No. ABIN7655693

The Rabbit Monoclonal anti-IL3RA antibody has been validated for IHC and IHC (fp). It is suitable to detect IL3RA in samples from Human.

Quick Overview for Recombinant IL3RA antibody (AA 26-171) (CF®568) (ABIN7655693)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IL3RA antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: IL3RA-2947R

Product Details anti-IL3RA Antibody (CF®568)

Binding Specificity: AA 26-171

Purpose: IL3RA / CD123 (Acute Myeloid Leukemia Marker)(IL3RA/2947R), CF568 conjugate

Characteristics: CD123 is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudo-autosomal region on chromosomes X or Y. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human IL3RA protein (around aa 26-171)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: THP-1, RPMI-8226 or HDLM-2 cells. Tonsil, Lymph Node or Stomach

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123

Background:

Synonyms: CD123, hIL3Ra, IL-3R-alpha, IL-3RA, IL3RAX, IL3RAY, IL3RX, IL3RY, Interleukin-3 Receptor, alpha, Interleukin3 receptor, Interleukin3 receptor, Y-chromosomal

Gene Symbol: IL3RA

Molecular Weight: 70 kDa

Gene ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signaling