IL-4 antibody (CF®488A)

Catalog No. ABIN7655723

The Rat Monoclonal anti-IL-4 antibody has been validated for ELISA, FACS and Func. It is suitable to detect IL-4 in samples from Mouse.

Quick Overview for IL-4 antibody (CF®488A) (ABIN7655723)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is conjugated to CF®488A

Application: ELISA, Flow Cytometry (FACS), Functional Studies (Func)

Clone: 11B11

Product Details anti-IL-4 Antibody (CF®488A)

Purpose: IL-4 (Interleukin-4), Mouse(11B11), CF488A conjugate

Characteristics: IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4R, and dendritic cell IL-12. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Partially Purified native Mouse IL-4

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Spleen, Thymus or Lymph Node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background:

Synonyms: B cell growth factor 1 (BCGF1), B cell IgG differentiation factor, B-Cell Stimulatory Factor 1 (BSF1), Binetrakin, IGG1 induction factor, IL4, Il4e12, Interleukin 4 variant 2, Interleukin 4, isoform 1, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra

Tissue Expression: Basophils|Mast cells|T-cells

Molecular Weight: 13-14 kDa

Gene ID: 16189

UniProt: P07750

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation