IL7R antibody (AA 21-236) (CF®647)

Catalog No. ABIN7655735

The Mouse Monoclonal anti-IL7R antibody has been validated for WB. It is suitable to detect IL7R in samples from Human.

Quick Overview for IL7R antibody (AA 21-236) (CF®647) (ABIN7655735)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL7R antibody is conjugated to CF®647

Application: Western Blotting (WB)

Clone: IL7R-2751

Product Details anti-IL7R Antibody (CF®647)

Binding Specificity: AA 21-236

Purpose: CD127 / IL7R (Marker of T-Reg Cells)(IL7R/2751), CF647 conjugate

Characteristics: The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 21-236) of human CD127 protein (IL7R)

Isotype: IgG2c, kappa

Application Details

Application Notes: ELISA: For coating, order Ab without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: K562, HeLa, Daudi or Jurkat cells. Tonsil, Lymph Node or Spleen.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: CD127

Background:

Synonyms: CD127, IL-7 receptor subunit alpha, IL-7R-alpha, IL7R, IL7RA, IL7R alpha, ILRA, Interleukin 7 receptor alpha chain, Interleukin 7 receptor

Gene Symbol: IL7R

Molecular Weight: 52-72 kDa

Gene ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size