Iba1 antibody (AA 1-146) (CF®405S)

Catalog No. ABIN7655767

This Mouse Monoclonal antibody specifically detects Iba1 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Iba1 antibody (AA 1-146) (CF®405S) (ABIN7655767)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: AIF1-2493

Product Details anti-Iba1 Antibody (CF®405S)

Binding Specificity: AA 1-146

Purpose: AIF1 / Iba1 (Microglia Marker)(AIF1/2493), CF405S conjugate

Characteristics: Allograft Inflammatory Factor 1 (also known as AIF1 or IBA1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant human AIF1 protein (around aa 1-146)

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Lymph Node, Tonsil or Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: Allograft Inflammatory Factor 1

Background:

Synonyms: Allograft inflammatory factor 1, AIF1, IBA1

Gene Symbol: AIF1

Tissue Expression: Microglia|Monocytes/macrophages

Molecular Weight: 17 kDa

Gene ID: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration