Cytokeratin 5 antibody (AA 316-590) (CF®640R)

Catalog No. ABIN7656188

The Mouse Monoclonal anti-Cytokeratin 5 antibody has been validated for IHC and IHC (fp). It is suitable to detect Cytokeratin 5 in samples from Human.

Quick Overview for Cytokeratin 5 antibody (AA 316-590) (CF®640R) (ABIN7656188)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KRT5-3594

Product Details anti-Cytokeratin 5 Antibody (CF®640R)

Binding Specificity: AA 316-590

Purpose: Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker)(KRT5/3594), CF640R conjugate

Characteristics: This MAb recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express. Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MCF-7 or HeLa cells. Human tonsil, esophagus or bladder tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Keratin 5

Background:

Synonyms: 58 kDa Cytokeratin, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, Type-II Cytoskeletal 5, Type-II keratin Kb5

Gene Symbol: KRT5

Tissue Expression: Epidermal cells

Molecular Weight: 58 kDa

Gene ID: 3852

UniProt: P13647