LGALS1/Galectin 1 antibody (AA 12-108) (CF®740)

Catalog No. ABIN7656318

This anti-LGALS1/Galectin 1 antibody is a Mouse Monoclonal antibody detecting LGALS1/Galectin 1 in WB, IHC, IF and IHC (fp). Suitable for Human.

Quick Overview for LGALS1/Galectin 1 antibody (AA 12-108) (CF®740) (ABIN7656318)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGALS1/Galectin 1 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: GAL1-1831

Product Details anti-LGALS1/Galectin 1 Antibody (CF®740)

Binding Specificity: AA 12-108

Purpose: Galectin-1 / Human Placental Lactogen (hPL) (GAL1/1831), CF740 conjugate

Characteristics: Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Galectin-1 protein fragment (aa12-108)

Isotype: IgG1, lambda

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. ELISA: 2-4 μg/mL for coating order Ab without BSA. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1

Background:

Synonyms: 14 kDa laminin-binding protein, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, HLBP14, HPL, Lactose-binding lectin 1, Lect14, Lectin galactoside-binding soluble 1, LGALS1, MAPK activating protein MP12, S-Lac lectin 1

Gene Symbol: LGALS1

Molecular Weight: 14 kDa

Gene ID: 3956

UniProt: P09382

Pathways: Carbohydrate Homeostasis