LIG1 antibody (CF®640R)

Catalog No. ABIN7656371

The Mouse Monoclonal anti-LIG1 antibody has been validated for IP, FACS and Func. It is suitable to detect LIG1 in samples from Human, Rat, Mouse and Cow.

Quick Overview for LIG1 antibody (CF®640R) (ABIN7656371)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG1 antibody is conjugated to CF®640R

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Functional Studies (Func)

Clone: 1A9

Product Details anti-LIG1 Antibody (CF®640R)

Purpose: DNA Ligase 1(1A9), CF640R conjugate

Characteristics: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Bloom's syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Full-length native cow LIG1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Western blot 0.5-1 μg/mL, ELISA, Immunoprecipitation, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA Ligase 1

Background:

Synonyms: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1

Gene Symbol: LIG1

Molecular Weight: 133 kDa

Gene ID: 3978

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA