MME antibody (CF®594)

Catalog No. ABIN7656865

This Mouse Monoclonal antibody specifically detects MME in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for MME antibody (CF®594) (ABIN7656865)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MME antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MME-1620

Product Details anti-MME Antibody (CF®594)

Purpose: CD10 (Membrane Metalloendopeptidase)(MME/1620), CF594 conjugate

Characteristics: CD10 (also known as Common Acute Lymphocytic Leukemia Antigen (CALLA)) is a type II transmembrane protein. It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CD10 protein (MME)

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 1-2 μg/million cells, Western blot 0.5-1 μg/mL, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Ramos cells (FACS/IF). Jurkat or LnCap lysates (WB). Kidney (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: CD10

Background:

Synonyms: CD10, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen (CALLA), Enkephalinase, gp100, Membrane metalloendopeptidase, Neutral endopeptidase

Gene Symbol: MME

Tissue Expression: B-cells|Epithelial cells

Molecular Weight: 100 kDa

Gene ID: 4311

UniProt: P08473

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration