Mesothelin antibody (AA 273-407) (Biotin)

Catalog No. ABIN7656966

This anti-Mesothelin antibody is a Mouse Monoclonal antibody detecting Mesothelin in IHC and IHC (fp). Suitable for Human, Mouse and Rat.

Quick Overview for Mesothelin antibody (AA 273-407) (Biotin) (ABIN7656966)

Target: Mesothelin (MSLN)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mesothelin antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MSLN-2131

Product Details anti-Mesothelin Antibody (Biotin)

Binding Specificity: AA 273-407

Purpose: Mesothelin (Mesothelial Marker) (MSLN/2131), Biotin conjugate

Characteristics: Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: CAPAN-2 or HeLa cells. Lung Mesothelioma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background:

Synonyms: CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP

Gene Symbol: MSLN

Molecular Weight: 40 kDa (Mature form), 69 kDa (Precursor)

Gene ID: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process