Recombinant HLA DQ antibody

Catalog No. ABIN7657100

The Mouse Monoclonal anti-HLA DQ antibody is suitable to detect HLA DQ in samples from Human and Pig. It has been validated for IP, WB and Func.

Quick Overview for Recombinant HLA DQ antibody (ABIN7657100)

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Antibody Type: Recombinant Antibody

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA DQ antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Clone: RSPV-L3

Product Details anti-HLA DQ Antibody

Purpose: HLA-DQ (MHC II)(rSPV-L3)

Characteristics: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: T4-positive CTL clone HG-38

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Raji cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for HLA DQ

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Alternative Name: HLA-DQ

Background:

Synonyms: CELIAC1, DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE), HLA class II histocompatibility antigen DQ(1) alpha chain, HLA class II histocompatibility antigen DQ(3) alpha chain, IDDM1, Leucocyte antigen DQA1, Major histocompatibility complex class II DQ alpha 1 (HLA DQA1), Major histocompatibility complex class II DQ beta 1 (HLA DQB1), MHC class II antigen HLA DQ beta 1, MHC class II HLA DQ alpha 1, MHC class II HLA DQ beta glycoprotein

Gene Symbol: HLA-DQ

Molecular Weight: 60 kDa

Gene ID: 3117, 3118, 3119

UniProt: P01908, P01909, P01920