HLA DQ antibody

Catalog No. ABIN6939631

The Mouse Monoclonal anti-HLA DQ antibody (Clone SPV-L3) (ABIN6939631) specifically detects HLA DQ in FACS and IF. The antibody is reactive with Human and Pig samples.

Quick Overview for HLA DQ antibody (ABIN6939631)

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA DQ antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPV-L3

Product Details anti-HLA DQ Antibody

Purpose: HLA-DQ (MHC II) Antibody

Specificity: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: T4-positive CTL clone HG-38

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HLA DQ

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Alternative Name: HLA-DQ

Background:

CELIAC1, DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE), HLA Class II DQ, HLA class II histocompatibility antigen DQ, HLA class II histocompatibility antigen DQ(1) alpha chain, HLA class II histocompatibility antigen DQ(3) alpha chain, HLA DQA1, HLA DQB, HLA DQB1, IDDM1, Leucocyte antigen DQA1, Major histocompatibility complex class II DQ alpha 1, Major histocompatibility complex class II DQ beta 1, MHC class II antigen HLA DQ beta 1, MHC class II HLA DQ alpha 1, MHC class II HLA DQ beta glycoprotein, MHC DQ beta

Raji cells. Tonsil or lymph node.

Molecular Weight: 60kDa

Gene ID: 3117, 3118, 3119

UniProt: P01908