Recombinant MYH11 antibody (CF®568)

Catalog No. ABIN7657675

This Rabbit Monoclonal antibody specifically detects MYH11 in IHC and IHC (fp). It exhibits reactivity toward Human, Rat, Cat, Chicken, Cow, Dog, Guinea Pig, Pig and Rabbit.

Quick Overview for Recombinant MYH11 antibody (CF®568) (ABIN7657675)

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Cat, Chicken, Cow, Dog, Guinea Pig, Pig, Rabbit

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYH11 antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MYH11-2303R

Product Details anti-MYH11 Antibody (CF®568)

Purpose: Smooth Muscle Myosin Heavy Chain (SM-MHC) (MYH11/2303R), CF568 conjugate

Characteristics: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human MYH11 protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Uterus or normal breast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Alternative Name: Smooth Muscle Myosin Heavy Chain

Background:

Synonyms: AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC

Gene Symbol: MYH11

Tissue Expression: Smooth muscle

Molecular Weight: 205 kDa (MHC-1) and 200 kDa (MHC-2)

Gene ID: 4629

UniProt: P35749