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Recombinant CD56 antibody (CF®647)

The Rabbit Monoclonal anti-CD56 antibody has been validated for FACS, IHC and IHC (fp). It is suitable to detect CD56 in samples from Human.
Catalog No. ABIN7657823

Quick Overview for Recombinant CD56 antibody (CF®647) (ABIN7657823)

Target

See all CD56 (NCAM1) Antibodies
CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This CD56 antibody is conjugated to CF®647

Application

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone

NCAM1-2217R
  • Purpose

    CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) (NCAM1/2217R), CF647 conjugate

    Characteristics

    This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Recombinant full-length human NCAM1 (CD56) protein

    Isotype

    IgG
  • Application Notes

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL

    Comment

    Positive Control: Colon or Small Intestine

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.1 mg/mL

    Buffer

    PBS, 0.1 % BSA, 0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light

    Storage

    4 °C

    Storage Comment

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    Expiry Date

    24 months
  • Target

    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

    Alternative Name

    CD56

    Background

    Synonyms: NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule

    Gene Symbol: NCAM1

    Tissue Expression: Skeletal Muscle|Neurons|NK cells

    Molecular Weight

    180, 145 and 125 kDa

    Gene ID

    4684

    UniProt

    P13591, P13592
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