Nkx2-2 antibody (AA 1-119) (CF®405S)

Catalog No. ABIN7657934

The Rabbit Monoclonal anti-Nkx2-2 antibody has been validated for IHC and IHC (fp). It is suitable to detect Nkx2-2 in samples from Human.

Quick Overview for Nkx2-2 antibody (AA 1-119) (CF®405S) (ABIN7657934)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NX2-2198R

Product Details anti-Nkx2-2 Antibody (CF®405S)

Binding Specificity: AA 1-119

Purpose: NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker)(NX2/2198R), CF405S conjugate

Characteristics: Expression of NKX2. 2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2. 2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human NKX2.2 protein fragment (around aa1-119)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Pancreas or Ewing sarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background:

Synonyms: Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman

Gene Symbol: NKX2-2

Molecular Weight: 40-50 kDa

Gene ID: 4821

UniProt: O95096

Pathways: Dopaminergic Neurogenesis