NRF1 antibody (Biotin)

Catalog No. ABIN7658105

The Mouse Monoclonal anti-NRF1 antibody has been validated for IHC and IHC (fp). It is suitable to detect NRF1 in samples from Human.

Quick Overview for NRF1 antibody (Biotin) (ABIN7658105)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NRF1 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NRF1-2608

Product Details anti-NRF1 Antibody (Biotin)

Purpose: NRF1(NRF1/2608), Biotin conjugate

Characteristics: The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human NRF1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Ubiquitous expression, strongest in skeletal muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1

Background:

Synonyms: alpha pal, Alpha palindromic-binding protein, Alpha-pal, locus control region factor 1, NFE2 related factor 1, Nuclear respiratory factor 1 (NFR-1)

Gene Symbol: NFE2L1

Molecular Weight: 30 kDa (bZIP region), 65-120 kDa (glycosylated)

Gene ID: 4899

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha