Nucleolin antibody (CF®740)

Catalog No. ABIN7658114

The Mouse Monoclonal anti-Nucleolin antibody has been validated for WB, IHC, IF, FACS and IHC (fp). It is suitable to detect Nucleolin in samples from Human.

Quick Overview for Nucleolin antibody (CF®740) (ABIN7658114)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 364-5 NCL-902

Product Details anti-Nucleolin Antibody (CF®740)

Purpose: Nucleolin(364-5 + NCL/902), CF740 conjugate

Characteristics: Recognizes Nucleolin (NCL), the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11 kb. It has a predicted molecular weight of ~76 kDa with an apparent MW of 100-110 kDa, attributed to phosphorylation of its N-terminal domain. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Lysate of SU-DHL-1 Nuclei (364-5), Recombinant human NCL protein (NCL/902)

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with mouse, rat or cow, others not known. Immunohistology (formalin). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin's lymphoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background:

Synonyms: NCL, Nucl, Nucleolin, Protein C23

Gene Symbol: NCL

Molecular Weight: 76 kDa (predicted), 100-110 kDa (apparent)

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization