Occludin antibody (AA 282-415) (CF®647)

Catalog No. ABIN7658199

This anti-Occludin antibody is a Mouse Monoclonal antibody detecting Occludin in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for Occludin antibody (AA 282-415) (CF®647) (ABIN7658199)

Target: Occludin (OCLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: OCLN-2181

Product Details anti-Occludin Antibody (CF®647)

Binding Specificity: AA 282-415

Purpose: Occludin (Marker of Early Blood Brain Barrier Damage)(OCLN/2181), CF647 conjugate

Characteristics: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Occludin protein (around aa 282-415)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HepG2 cells. Kidney.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin

Background:

Synonyms: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin

Gene Symbol: OCLN

Tissue Expression: Microvascular endothelial cells

Molecular Weight: 60-82 kDa

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C